Axons of dopaminergic neurons express gamma-aminobutyric acid type-A receptors (GABARs) and nicotinic acetylcholine receptors (nAChRs), which are positioned to shape striatal dopamine release. We examine how interactions between GABARs and nAChRs influence dopaminergic axon excitability. Axonal patch-clamp recordings reveal that potentiation of GABARs by benzodiazepines suppress dopaminergic axon responses to cholinergic interneuron transmission. In imaging experiments, we use the first temporal derivative of axonal calcium signals to distinguish between direct stimulation of dopaminergic axons and nAChR-evoked activity. Inhibition of GABARs with gabazine selectively enhance nAChR-evoked axonal calcium signals but does not alter the strength or dynamics of acetylcholine release, suggesting that the enhancement is mediated primarily by GABARs on dopaminergic axons. Unexpectedly, we find that a widely used GABAR antagonist, picrotoxin, inhibits axonal nAChRs and should be used cautiously for striatal circuit analysis. Overall, we demonstrate that GABARs on dopaminergic axons regulate integration of nicotinic input to shape axonal excitability.